Thyrotropine releasing hormone accelerates wound healing in hyperglycemia inducted mouse fibroblast cell line

Background and Aims: It has been suggested that thyrotropin-releasing hormone (TRH) may have functions beyond its fundamental regulating function. Previous studies demonstrated TRH promotes wound healing. We aimed to perform an in vitro study fibroblasts assess the role of wounds frequently occur diabetes. Thus, we investigated effects healing both under normoglycemic hyperglycemic-conditions. Methods: L929 mouse fibroblast cell line was used experiments. The viability confirmed with XTT. Then scratch migration assay for assessing added control hyperglycemia groups at 100 nM after created. areas were measured 24 48 hours scratch. Results: and/or did not affect cellular activity hours. reduced (42.6%) compared (52.2%) In group area 64.3% 61.0% initial 24th 48th respectively. incubation these 55.2% 47.1% areas. Conclusion: treatment accelerated hyperglycemia, which indicates positive wounds,